Sterilization

Sterilization Instrument Care

Material Suggested Care
Stainless Steel Instruments While stainless steel has superior corrosion resistance, it will discolor and
corrode when exposed to higher than recommended chemical concentrations or
certain chemicals. Stainless steel should not be exposed to the following
chemicals: Sodium Hypochlorite (household bleach), Tartaric acid (stain and
tartar remover), Aluminum Chloride, Barium Chloride, Bichloride of Mercury,
Calcium Chloride, Carbolic Acid, Chlorinated Lime, Citric Acid, Dakin’s
Solution, Ferrous Chloride, Lysol, Mercuric Chloride, Mercury Salts, Phenol,
Potassium Permanganate, Potassium, Thiocyanate, or Stannous Chloride.

The following chemicals should NEVER be used with stainless
steel: Aqua Regia, Ferric Chloride, Sulfuric Acid, Hydrochloric Acid or Iodine.

Carbon Steel Instruments Carbon steel instruments are more sensitive to chemicals than stainless steel
and require special handling. Carbon steel should NOT be
exposed to any of the previously listed chemicals for stainless steel.

Separate carbon steel instruments from stainless steel instruments throughout
the cleaning and sterilization process. If processed together, the carbon steel
instruments will likely create cross-contamination on the stainless
instruments.

Carbon steel instruments should be thoroughly dried prior to sterilization to
prevent rusting and/or corrosion. Use a protective rust-inhibitor before
sterilization.

Hinged Instruments All hinged instruments—Forceps, ronguers, scissors, pliers, hemostats,
orthodontic pliers, etc.—should be kept lubricated. Regular use of proper
lubricants, like Instrument Lubricant, will prevent rust, corrosion and stiff joints
and will ensure smooth operation. (Household lubricants and hand piece
lubricants are NOT recommended). All hinged instruments should be sterilized
in the open position
Anodized Aluminum Special care needs to be exercised in cleaning and sterilizing these coated
aluminum instruments. Do not clean in an ultrasonic unit. Clean by hand or in
an automated washer. Check processing product labels for caution about use
with aluminum. Sterilize in autoclave, chemiclave or dry heat under 350°F
(177°C) according to manufacturer’s instructions. Note: Anodized aluminum
instruments, when sterilized with stainless steel instruments, may cause an
adverse chemical reaction.
Ultrasonic Inserts To clean, fully immerse in a mild ultrasonic cleaning solution (pH 7-10.5). Daily
clean or place in Ultrasonic unit for 7 to 10 minutes or 16 to 20 minutes if the
inserts are in a cassette. After ultrasonic cleaning, only use distilled water for
rinsing- do not use tap water. We recommend “Steri-shine” solution as it does
not require rinsing after ultrasonic.

Problem Solving for Instruments

Problem Reason Prevention
Spotting
  • Insufficient drying after ultrasonic cleaning
  • Not changing ultrasonic solution
  • Sterilizer has not been cleaned
  • Solution should be changed at least once a day
  • Sterilizers should be cleaned weekly
  • Optional: Dip cassettes in alcohol after rinsing
Rust
  • Corrosion from carbon instruments spreads to stainless steel instruments
  • Separate stainless and carbon instruments
  • For carbon steel instruments: Dip in pre-sterilized
    rust-inhibiting solution as suggested by sterilizer manufacturer
Pitting
  • Chemical attack on instruments
  • Rinse and dry instruments thoroughly
  • Use approved cleaning, sterilization solutions only
  • Never use household bleach or stain and tartar remover

Problem Solving for Cassettes

Problem Reason Prevention
Staining
  • Amalgam left in carrier
  • Chrome breakdown of instruments
  • Sterilizer has not been cleaned
  • Thoroughly empty amalgam carrier before returning to cassette
  • If carrier is plugged, sterilize separately and unplug while carrier is warm
  • Inspect instruments and replace those with cracked handles or peeling plating
  • Change reservoir water once a week
  • Regularly clean chamber and filters
Broken Hinges
  • Overloading / improperly placed instruments
  • Improperly placed rails
  • Instruments should not protrude from cassette
  • Only light force is needed to close cassette
  • Do not reposition cassette rails
Wet Packs
  • Insufficient drying either before or during sterilization
  • Improper loading of cassettes in sterilizer
  • Thoroughly dry cassettes after cleaning, before wrapping
  • Crack open autoclave sterilizer door during dry cycle
  • Optional: After sterilization cycle, leave cassettes in warm sterilizer for 10 minutes
  • Do not over pack sterilizer
  • Keep cassettes slightly separated within the chamber
  • Always use sterilizer’s cassette rack

Instrument Cleaning and Sterilization

Cleaning All instruments need to be cleaned and thoroughly dried before they are sterilized. They
should be washed with a non-corrosive, low suds, neutral detergent, instrument cleaning
can be accomplished by ultrasonic or automated cleaning, which is preferred to minimize
the opportunity of sharps injuries due to hand scrubbing.
Magnum Medical does not suggest the use of any abrasive brushes or materials to clean
instruments.
Sterilization Sterilization is a process that kills microorganisms. There are three common methods of
heat sterilization used in the dental office that can be verified by spore testing (steam
autoclave, dry heat, and chemical vapor)

Comparison of Cleaning Methods

Method Advantages Disadvantages
Hand Scrubbing
  • Effective if performed properly
  • Increases chances for operator injury
  • Increases spread of contamination through splatter
  • Labor-intensive
  • Need proper care of scrub brush
Ultrasonic Cleaning
  • Safer than hand scrubbing
  • Effectively cleans all instruments
  • Reduces chances for spread of contaminants through splatter
  • Allows for more efficient use of staff time
  • Microorganisms may accumulate in cleaning solution
  • Ultrasonic cleaning will not remove hardened permanent cement
    (Solution: remove cement while it is still soft)

Comparison of Heat Sterilization Methods

(These conditions do not include warm-up time and they may vary depending on the nature and volume of the load)

Method Sterilization Advantages Precautions
Steam Autoclave 20 minutes at
250°F / 121°C
(15psi)
  • Time efficient
  • Good penetration
  • Sterilize water-based liquid
  • Do not use closed containers
  • May damage plastic and rubber items
  • Non-stainless steel metal items corrode
  • Use of hard water may leave deposits
  • Dry instruments
Unsaturated Chemical Vapor 20 minutes at
270°F / 132°C (20–40 psi)
  • Time efficient
  • No corrosion
  • Items dry quickly after cycle
  • Do not use closed containers
  • May damage plastic and rubber items
  • Must use special solution
  • Dry instruments or dip in special solution
  • Provide adequate ventilation
  • Cannot sterilize liquids
Dry Heat Oven 60–120 minutes at
320°F / 160°C
  • No corrosion
  • Can use closed containers
  • Large capacity per cost
  • Items are dry after cycle
  • Longer sterilization time
  • Cannot sterilize liquids
  • May damage plastic and rubber items
  • Do not open door before end of cycle
  • Dry instruments
Rapid Dry Heat Transfer 12 minutes at 350°F / 177°C (wrapped items)

6 minutes at 350°F / 177°C (unwrapped items)

  • No corrosion
  • Short cycle
  • Items are dry after cycle
  • Cannot sterilize liquids
  • May damage plastic and rubber items
  • Do not open door before end of cycle
  • Small capacity per cost
  • Dry instruments
  • Unwrapped items become contaminated after cycle

Precautions about the use of chemicals for instrument sterilization

Chemicals not to be used for stainless steel instruments and T/C steel instruments: Aqua Regia,
Ferric Chloride, Sulfuric Acid, Hydrochloric Acid, Sodium Hypochlorite, Tartaric Acid, Aluminum
Chloride, barium Chloride of Mercury, Calcium Chloride, Carbolic Acid, Chlorinated Lime, Citric
Acid, Dakin’s Solution, Ferrous Chloride, Lysol, Mercuric Chloride, Mercury Salts, Phenol,
Potassium Permanganate, Stannous Chloride.

Do NOT mix T/C steel instruments with stainless steel instruments during the cleaning and
sterilization, which could cause cross-corrosion on the stainless instruments.

Resin instruments are to be sterilized with ultrasonic or automated cleaning and steaming. It is
not recommended to sterilize it with dry heat or rapid dry heat transfer.

Anodized Aluminum is well maintained with autoclave, chemiclave or dry heat, but in ultrasonic
units.

Recommended Method of Instruments Sterilization

Sterilization Method Recommended
Temperature
Expected Advantage
Steam Autoclave
  • 121°C for 120 minutes
  • Time Efficient
  • Good Penetration
  • Sterilize water-based
    liquid
Dry Heat
  • 160°C for 60 to 120 minutes
  • No Corrosion
  • Large capacity per cost
  • Items are dry after cycle
Chemical Vapor
  • 132°C for 20 minutes
  • Time Efficient
  • No Corrosion
  • Items dry quickly after
    cycle